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Were there other superheroes that existed in the Spectacular Spider-Man universe? Like, Avengers, Fantastic Four, X-Men etc.
In theory, yes.
Not the Avengers, not yet. (If you mean as a team. Some of the individual members existed.)
The Fantastic Four were in business.
The X-Men had also not yet been formalized as a team. Though Professor X had recently opened his school.
I've answered this in more detail previously. Check the archives.